Measurement of cell kinetics in cervical tumours using bromodeoxyuridine
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منابع مشابه
Measurement of T and B cell turnover with bromodeoxyuridine.
This unit describes two methods used to determine the rate of turnover of T and B cells in vivo. In each method, this is accomplished by administering 5-bromo-2'-deoxyuridine (BrdU), a thymidine analog, and measuring the rate at which cells become labeled with this DNA precursor during the S phase of the cell cycle. Cells that contain BrdU are then detected in vitro using fluorochrome-labelled ...
متن کاملCell Kinetics in Human Malignancies Studied with in Vivo Administration of Bromodeoxyuridine and Flow Cytometry1
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incor porated into the DN A of proliferating cells. When in vivoBrdUrd infusion is coupled with Divariate flow cytomctry to measure cell BrdUrd incor poration and I)V\ content, both the percentage of DNA-synthesizing cells jBrdUrd-labeling index (LI)] and the DNA synthesis time (Ts) can be determined on the same tissue sample. From ex...
متن کاملCell kinetics in human malignancies studied with in vivo administration of bromodeoxyuridine and flow cytometry.
Bromodeoxyuridine (BrdUrd) is a pyrimidine analogue which is incorporated into the DNA of proliferating cells. When in vivo BrdUrd infusion is coupled with bivariate flow cytometry to measure cell BrdUrd incorporation and DNA content, both the percentage of DNA-synthesizing cells [BrdUrd-labeling index (LI)] and the DNA synthesis time (TS) can be determined on the same tissue sample. From exper...
متن کاملMeasurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine.
In vivo bromodeoxyuridine (BrdUrd) labelling of the human large bowel was performed and a detailed histochemical localisation of label in sections of crypts was undertaken using a monoclonal antibody to BrdUrd containing DNA. Flow cytometric studies on extracted nuclei were also performed (data presented elsewhere). The average crypt in the human large bowel (excluding the rectum) was 82 cells ...
متن کاملGut, 1992,33,71-78 Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine
In vivo bromodeoxyuridine (BrdUrd) labelling of the human large bowel was performed and a detailed histochemical localisation of label in' sections of crypts was undertaken using a monoclonal antibody to BrdUrd containing DNA. Flow cytometric studies on extracted nuclei were also performed (data presented elsewhere). The average crypt in the human large bowel (excluding the rectum) was 82 cells...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1993
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1993.307